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. 2022 Dec 7;11(1):e03515-22. doi: 10.1128/spectrum.03515-22

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Copyright © 2022 Wen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — NbBAK1 and NbSOBIR1 were required for VdGAL4-induced cell death. (A) Virus-induced gene silencing (VIGS) assay was used to silence NbBAK1 and NbSOBIR1 by inoculation with TRV vectors (pTRV2-GFP, pTRV2-NbBAK1, pTRV2-NbSOBIR1) in N. benthamiana. Three weeks after inoculation, pGR107-VdGAL4, pGR107-BAX, and pGR107-GFP were transiently expressed in NbBAK1- and NbSOBIR1-silenced N. benthamiana leaves. The photographs were taken 7 dpa. (B) Expression levels of NbBAK1 and NbSOBIR1 after VIGS treatment by RT-qPCR. NbActin was used as the internal reference gene. (C) Western blot analysis of transient expression proteins of VdGAL4. Proteins were stained with Coomassie brilliant blue (CBB) to determine equal loading. **, P < 0.01.