Assays of the role of VdGAL4 in penetration of mycelium. (A) Cellophane membranes were plated onto PDA and inoculated with conidia suspension from WT, ΔVdGAL4, and C-ΔVdGAL4 strains and grown on cellophane for 3 days. (A, Bottom) Indicated cellophane was removed for 3 days. (B) Micromorphology of mycelium of all tested strains observed on cellophane by scanning electron microscope. Bar, 200 μm. (C) Assays of the relative expression of conidia-related genes in all tested strains by RT-qPCR. The error bar represents standard error of the mean. **, P < 0.01.