FIG 1.
HBx downregulates BANF1 expression in hepatoma cells. Huh-7, Hep G2, and Hep G2.2.15 cells were cultured, and the total RNA was isolated followed by RT-PCR for BANF1 expression (n = 3; **, P < 0.01) (A). Huh-7 cells were transfected with pHBV1.3 plasmid and the cells were collected for the isolation of RNA, followed by RT-PCR for HBx (B) or BANF1 (C) expression (n = 3; ***, P < 0.001; **, P < 0.01). Huh-7 cells were transfected with HBsAg, HBcAg and HBV-pol plasmid, total RNA was isolated and RT-PCR was performed for HBsAg, HBcAg and HBV-pol (n = 3; *, P < 0.05; **, P < 0.01) (H) and BANF1 (I). After 48 h, both the RNA and protein were isolated and RT-PCR or Western blots were performed. The expression of HBx (D) or BANF1 (E) was determined (n = 3; ***, P < 0.001; **, P < 0.01). The total cellular protein was run on SDS-PAGE gels and Western blots were performed and a representative picture is shown. Lane 1, control; lane 2, Empty vector transfected cells; and lane 3, HBx-transfected cells (F). The band intensities were quantified using Image J software and presented as fold change (BANF1/β-actin) n = 3; **, P < 0.01 (G).