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. 2022 Dec 15;11(1):e01235-22. doi: 10.1128/spectrum.01235-22

FIG 3.

FIG 3

Mutation at the N-terminus of BANF1 modulates the expression of HBx, HBsAg and HBV replication. Huh-7 cells were transfected either alone with pHBV1.3 plasmid or cotransfected with BANF1 (WT), M1, M2, or M3 plasmid. After 48 h cells were harvested for total cellular protein isolation and Western blots were performed. A representative picture is shown. Lane 1, control; lane 2, empty vector transfected cells; lane 3, BANF1 transfected cells; lane 4, pHBV1.3 transfected cells; lane 5, BANF1(WT) and pHBV1.3 cotransfected cells; lane 6, M1 and pHBV1.3 cotransfected cells; lane 7, M2 and pHBV1.3 cotransfected cells; lane 8, M3 and pHBV1.3 cotransfected cells (A). The band intensities were quantified using Image J software and presented as fold change (HBx/β-actin, BANF1/β-actin) (n = 3; *, P < 0.05) (B). Hep G2.2.15 cells were transfected with BANF1 (WT), M1, M2, and M3 plasmid and after 48 h media was collected for HBsAg analysis by Enzyme Linked Fluorescent Assay (ELFA) (n = 3; *, P < 0.05) (C) and cells were harvested for HBV DNA isolation and quantification by qPCR (n = 3; ***, P < 0.001) (D).