FIG 2.

Optimal reaction conditions of the P. aeruginosa CRISPR-top assay. Error bars represent the means ± standard error of means (SEM); n = 3 technical replicates. (a) Optimal reaction temperature. The reactions were performed under temperatures ranging from 53°C to 58°C at 1°C intervals. (b) Optimal primer concentration. Serial volumes of LAMP primer premixture (0.3 to 2.1 μL at 0.3-μL intervals) were used to prepare the reaction mixtures. (c) Optimal ssDNA reporter molecule concentration. Serial volumes of 100 μM ssDNA probe (0.2 to 2.0 μL at 0.3-μL intervals) were used to prepare the reaction mixtures.