FIG 3.

Specificity of the P. aeruginosa CRISPR-top assay. PC, positive control (P. aeruginosa ATCC 27853); NC, negative control (DW). 1, Klebsiella pneumoniae; 2, Acinetobacter baumannii; 3, Streptococcus pneumoniae; 4, Moraxella catarrhalis; 5, Staphylococcus aureus; 6, Escherichia coli; 7, Mycobacterium tuberculosis; 8, Klebsiella aerogenes; 9, Klebsiella oxytoca; 10, Serratia marcescens; 11, Acinetobacter pizzerialum; 12, Acinetobacter junii; 13, Streptococcus mitis; 14, Streptococcus agalactiae; 15, Streptococcus pyogenes; 16, Streptococcus salivarius; 17, Streptococcus oralis; 18, Streptococcus suis; 19, Staphylococcus haemolyticus; 20, Staphylococcus succinus; 21, Staphylococcus epidermidis; 22, Stenotrophomonas maltophilia; 23, Nocardia cyriacigeorgica; 24, Nocardia farcinica; 25, Corynebacterium striatum; 26, Corynebacterium simulans; 27, Corynebacterium propinquum; 28, Corynebacterium aurimucosum; 29, Enterococcus faecalis; 30, Aeromonas caviae; 31, Elizabethkingia anophelis; 32, Ralstonia mannitolilytica; 33, Rothia kristinae; 34 to 48, P. aeruginosa clinical isolates. Error bars represent means ± SEM (n = 3 technical replicates).