TABLE 1.
Primers, gRNA, and ssDNA reporter molecules used in this study
| Primer | Type | Sequence (5′–3′)a | Length |
|---|---|---|---|
| Pa-F3 | Forward outer primer | CGCGTAGTGCTGGAAGG | 17 nt |
| Pa-B3 | Backward outer primer | GGTTCTGAGCCCAGGACTG | 19 nt |
| Pa-FIP | Forward inner primer | TAGCGCTGAACGGCCTTGTTCGGCACCCGCGAGTACA | 37 nt |
| Pa-BIP | Backward inner primer | GCAGGGTGTTTCGCCGTCGTCGTGGCCGGTAG | 32 nt |
| Pa-LF | Loop forward primer | GCACGACGCTCGCCCAGAG | 19 nt |
| Pa-LB | Loop backward primer | GGTAAAGAGCGTCCGGTCG | 19 nt |
| gRNA | Guide RNA | GUCUAGAGGACAGAAUUUUUCAACGGGUGUGCCAAUGGCCACUUUCCAGGUGGCAAAGCCCGUUGAGCUUCUCAAAUCUGAGAAGUGGCACGGCACCCGCGAGUACAAUAU | 111 nt |
| ssDNA reporter molecule | ssDNA reporter molecule | 6-FAM-TTATTATTAT-BHQ1 | 10 nt |
a
The inserted PAM site (TTC) is in bold.