TABLE 1.
Mutation calls (whether walK 646C>G was called) by BWA/BCFtools and Medaka for simulated, amplicon DNA, and culture mixtures
| Proportion N384/N384-3 | Simulated (NanoSim) |
Amplicon DNA |
Culture |
|||
|---|---|---|---|---|---|---|
| BWA/BCFtools | Medaka | BWA/BCFtools | Medaka | BWA/BCFtools | Medaka | |
| 100% N384 | No | No | No | No | No | No |
| 90% N384 | No | No | Yes | No | Yes | No |
| 10% N384-3 | ||||||
| 80% N384 | No | No | ||||
| 20% N384-3 | ||||||
| 70% N384 | No | No | ||||
| 30% N384-3 | ||||||
| 60% N384 | No | No | ||||
| 40% N384-3 | ||||||
| 50% N384 | Yes | No | Yes | Yes | Yes | No |
| 50% N384-3 | ||||||
| 40% N384 | Yes | Yes | ||||
| 60% N384-3 | ||||||
| 30% N384 | Yes | Yes | ||||
| 70% N384-3 | ||||||
| 20% N384 | Yes | Yes | ||||
| 80% N384-3 | ||||||
| 10% N384 | Yes | Yes | Yes | Yes | Yes | Yes |
| 90% N384-3 | ||||||
| 100% N384-3 | Yes | Yes | Yes | Yes | Yes | Yes |