TABLE 6.
Summary of the primers and probes designed to target variant-specific mutations (VSM) of the Beta, Gamma, Delta, and Kappa variants of SARS-CoV-2
| Target variant | Target VSM | Oligo type | Sequencea |
|---|---|---|---|
| Beta (B.1.315) | K417N | Forward primer | TGAAGTCAGACAAATCGCTCC |
| Reverse primer | CAAGCTATAACGCAGCCTGT | ||
| Probe | HEX-AGGGCAAACTGGAAATATTGCTG-BHQ | ||
| Gamma (P1) | K417T | Forward primer | TGAAGTCAGACAAATCGCTCC |
| Reverse primer | CAAGCTATAACGCAGCCTGT | ||
| Probe | FAM-ACTGGAACGATTGCTGATTATAATT-MGB | ||
| Delta (B.1.617.2) | 156-157DEL | Forward primer | GATCCATTTTTGGGTGTTTATTACC |
| Reverse primer | GGCTGAGAGACATATTCAAAAGTG | ||
| Probe | FAM-TGGAAAGTAGAGTTTATTCTAGTGCG-MGB | ||
| Kappa (B.1.617.1) including B.1.617.3) | E154K | Forward primer | GCCGGTAGCACACCTTGTAA |
| Reverse primer | GTTGGAAACCATATGATTGTAAAGGA | ||
| Probe | HEX-TGGTGTTCAAGGTTTTAATTGTTAC-BHQ |
a
The dye names are present in bold.