FIG 1.

Φ1207.3 induction by mitomycin C. To determine if Φ1207.3 has a lytic phenotype, mitomycin C was added at a subinhibitory concentration (100 ng/mL) to liquid bacterial cultures in early exponential phase. (A) In the Φ1207.3-carrying strain FR1, mitomycin C causes a limited phage burst and a growth impairment, resulting in early entrance in the stationary phase (2.4 generations before of the untreated culture). (B) In the FP10 parental strain, mitomycin C produces only a slight growth impairment. The time point of mitomycin C addition is indicated by an arrow. Results are reported as means and standard deviations resulting from at least three independent experiments.