TABLE 2.
PCR quantification of the Φ1207.3 phage genome
| Samplea | Excised phages | mef(A) | gyrB |
|---|---|---|---|
| Bacterial culture | 2.70 × 105 ± 4.58 × 104 | 4.87 × 106 ± 3.34 × 106 | 5.50 × 106 ± 4.01 × 106 |
| Phage preparations | 1.03 × 107 ± 1.31 × 107 | 3.81 × 107 ± 4.05 × 107 | 3.91 × 105 ± 2.30 × 105 |
| Fold enrichment | 38 | 7.8 | 0.1 |
| Phage preparations (MitC+) | 1.87 × 107 ± 1.81 × 107 | 1.86 × 108 ± 2.13 × 108 | 9.18 × 105 ± 3.93 × 105 |
| Fold enrichment | 70 | 38 | 0.2 |
a
PCR starting templates were (i) the FR1 liquid culture, (ii) the phage preparations from the FR1 untreated culture, and (iii) the phage preparations from the FR1 mitomycin C-treated culture. Amplification target sequences were (i) the joints between the ends of Φ1207.3 sequence, i.e., the excised form of phage genome, (ii) the macrolide resistance gene mef(A) gene of Φ1207.3, and (iii) the chromosomal reference gene gyrB. Fold enrichment was calculated as the ratio between the value obtained from the phage preparation and from bacterial culture. Results are reported as means and standard deviations from 4 different experiments.