TABLE 3.
Plasmid subtypes and E. coli sequence types of human isolates obtained from farms without ESBL producer-harboring pig isolates
| Source | Sampling occasion | Gene | Plasmida | ST (no. of isolates) |
|---|---|---|---|---|
| Human (farm 11) | 6 mo | bla CTX-M-15 | Chromosome | 131 (1) |
| Human (farm 14) | 6 mo | bla CTX-M-1 | IncI1 (58/58) | 34 (1) |
| bla CTX-M-1 | IncI1 (58/58) | 23 (1) | ||
| Human (farm 15) | 6 mo | bla CTX-M-2 | IncI1 (12/12) | 58 (1) |
| Human (farm 17) | 12 mo | bla CTX-M-1 | IncI1 (3/3) | 23SLV (1) |
| Human (farm 23) | 0 mo | bla CTX-M-1 | F2:A-:B- | 86 (1) |
| bla CTX-M-1 | IncI1 (3/3) | 58 (1) | ||
| bla CTX-M-1 | IncI1 nontypeableb | 86 (1) |
a
For plasmid belonging to IncI1 group we report the plasmid sequence type (pST) and plasmid clonal complex (pCC) within parentheses as (pST/pCC).
b
Exact matches found only for 4 out of 5 loci included in the plasmid MLST scheme (repI1 [2]-ardA [1]-trbA [4]-pilL [2]), while no positive PCR was obtained for the sogS gene after repeated attempts. The partial allelic profile obtained could correspond to pST-3/pCC-3, pST-101, or pST-220 based on the IncI1 plasmid MLST database (https://pubmlst.org/bigsdb?db=pubmlst_plasmid_seqdef).