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Comparison of various parameters for the assays used in this research for detection of IBV
| Parameter | Sandwich ELAA | qRT-PCR | Homogeneous PLA | Solid-phase PLA |
|---|---|---|---|---|
| LOD (EID50/mL) | 1.2 | 0.6 | 0.6 | 0.5 |
| LLOQ (EID50/mL) | 10 | 0.5 | 0.3 | 0.1 |
| ULOD (EID50/mL) | 73 | 103 | 104 | 104 |
| MDD (EID50/mL) | 1 | 0.2 | 0.2 | 0.1 |
| Dynamic range | 103 | 106 | 107 | 108 |
