TABLE 1.
In vitro evaluation of the BLESSED protocol for the detection of 16 ESBL-PE strains spiked at 103 and 104 CFU/mL into calibrated swab liquid sample suspensions
| GNB strain | ESBL enzyme produced | CFU/mL | Enrichment factor (fold) | BLT result at: |
|||
|---|---|---|---|---|---|---|---|
| 15 min | 30 min | 45 min | 60 min | ||||
| Klebsiella pneumoniae | CTX-M-22 | 103 | 350 | − | − | + | + |
| 104 | 540 | + | + | + | + | ||
| Klebsiella pneumoniae | CTX-M-3 | 103 | 160 | − | − | − | − |
| 104 | 740 | + | + | + | + | ||
| Klebsiella pneumoniae | CTX-M-15 | 103 | 310 | + | + | + | + |
| 104 | 330 | + | + | + | + | ||
| Klebsiella pneumoniae | SHV-12 | 103 | 500 | − | − | − | − |
| 104 | 930 | − | + | + | + | ||
| Klebsiella pneumoniae | TEM-3 | 103 | 250 | − | − | − | − |
| 104 | 96 | − | + | + | + | ||
| Klebsiella pneumoniae | TEM-21 | 103 | 200 | − | − | − | − |
| 104 | 20 | + | + | + | + | ||
| Escherichia coli | CTX-M-27 | 103 | 43 | − | − | − | − |
| 104 | 7 | + | + | + | + | ||
| Escherichia coli | CTX-M-1 | 103 | 50 | + | + | + | + |
| 104 | 64 | − | − | + | + | ||
| Escherichia coli | SHV-2 | 103 | 75 | − | − | − | − |
| 104 | 93 | − | − | − | + | ||
| Citrobacter freundii | CTX-M-3 | 103 | 36 | − | − | − | − |
| 104 | 1.3 | − | − | + | + | ||
| Citrobacter freundii | TEM-3 | 103 | 5 | − | − | − | − |
| 104 | 24 | − | − | − | + | ||
| Enterobacter cloacae | CTX-M-15 | 103 | 2 | − | − | − | − |
| 104 | 5 | − | + | + | + | ||