TABLE 4.
Mutations introduced into genome sequence AE000516.2 CDC1551 in the synthetic FASTQ prepared for the 2020 panel
| Year | Sample | Gene | Mutation introduced (position in reference genome) | Frequency (%) | Interpretation |
|---|---|---|---|---|---|
| 2020 | 11 | rpoB | Ser450Leu C > T (761155) | 60 | Rifampicin-resistant |
| katG | Ser315Thr G > C (2155168) | 60 | Isoniazid-resistant | ||
| pncA | 14-bp deletion (2289219 to 2289232) | 40 | Pyrazinamide-resistant | ||
| nirB | Lys686Lys A > G (304923) | 100 | NA | ||
| 2020 | 12 | rpoB | Ser450Leu C > T (761155) | 100 | Rifampicin-resistant |
| katG | Ser315Thr G > C (2155168) | 100 | Isoniazid-resistant | ||
| pncA | 14-bp deletion (2289219 to 2289232) | 100 | Pyrazinamide-resistant | ||
| nirB | Lys686Asn A > G>C (304923) | 100 | NA | ||
| 2020 | 13 | rpoB | Ser450Leu C > T (761155) | 100 | Rifampicin-resistant |
| katG | Ser315Thr G > C (2155168) | 100 | Isoniazid-resistant | ||
| pncA | 14-bp deletion (2289219 to 2289232) | 100 | Pyrazinamide-resistant | ||
| ddn (Rv3547) | Trp88Stop G > A (3987106) | 100 | Possible delamanid resistance (27) | ||
| Rv0678 | Met1Ala T > C (778991), loss of start codon | 100 | Possible bedaquiline resistance (19) | ||
| nirB | Lys686Asn A > G>C (304923) | 100 | NA | ||
| Rv1985C | Pro34Pro G > C (2229801) | 100 | NA | ||
| Rv2845c (lipQ) | Arg60Leu C > A (2793810) | 100 | NA | ||
| glpK | Insertion CCC (4139190) | 100 | NA | ||
| Rv1592c | Ile322Leu T > G (1792777) and Glu321Asp T > A (1792778) | 100 | NA |