TABLE 2.
Optimization of RT-RPA-VF reaction temperature
| Amt of positive plasmid used as template (copies/μL) | Result with indicated reaction temp (°C) |
|||||
|---|---|---|---|---|---|---|
| 33 | 35 | 37 | 39 | 40 | 42 | |
| 3.6 × 101 | + | + | + | + | + | + |
| 3.6 × 100 | − | − | + | + | + | + |
| 3.6 × 10−1 | − | − | − | − | − | + |
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