TABLE 3.
Optimization of RT-RPA-VF reaction time
| Amt of positive plasmid used as template (copies/μL) | Result with indicated reaction time (min) |
||||
|---|---|---|---|---|---|
| 10 | 15 | 20 | 25 | 30 | |
| 3.6 × 101 | − | + | + | + | + |
| 3.6 × 100 | − | + | + | + | + |
| 3.6 × 10−1 | − | − | − | − | − |
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