TABLE 1.
Clinical isolates used in this studya
| Isolate | Sample origin | ST | Hypermutator | MIC (mg/L) |
Acquired resistome | Mutational resistome | ||
|---|---|---|---|---|---|---|---|---|
| CIP | ATM | TOB | ||||||
| CAN01-002 | Sputum | 111 | No | 0.032 | 0.38 | 2 | mexB (Q319X), mexY (G530S), mexZ (Q140K), mexT (G276D), oprD (nt174Δ11), mexS (nt300IS) | |
| CAT06-005 | Sputum | 175 | No | 16 | 4 | 48 | aadB | oprM (T198P), oprD (Q142X), mexZ (G195D), gyrA (T83I, D87N), ampR (G154R), parC (S87W), armZ (V266M) |
| MAD04-002 | Sputum | 242 | No | 0.19 | 6 | 2 | parC (K726R) | |
| AND04-004A | Endotracheal aspirate | 244 | No | 0.125 | 4 | 1 | ||
| CAT02-004 | Blood | 244 | No | 0.5 | 12 | 0.75 | mexR (nt266ins1), dacB (A340G) | |
| CAT09-004 | Blood | 244 | No | 0.094 | 3 | 1.5 | ||
| CAT09-003 | Sputum | 253 | No | 0.25 | 6 | ≥256 | aacA4 | mexZ (H51D) |
| ARA02-005 | Blood | 267 | No | 0.125 | 3 | 1 | oprN (R151L), armZ (R320C) | |
| FQSE10-0503 | CF sputum | 274 | No | 0.5 | 3 | 4 | mexY (V875M), mexZ (IS) | |
| FQSE15-0803 | CF sputum | 274 | Yes, mutS (nt814Δ4) | 0.38 | 0.38 | 3 | mexA (L338P), mexZ (A144V) | |
| PAMB148 | Blood | 274 | No | 0.094 | 96 | 4 | mexY (V875M), ampD (P41L) | |
| CLE03-004 | Endotracheal aspirate | 381 | No | 0.125 | 3 | 1 | mexZ (nt386Δ1) | |
| CVA02-003 | Sputum | 640 | No | 0.38 | 4 | 48 | gyrB (S466F), mexA (E53X), parS (S33R), mexY (T472P), mexZ (nt491Δ10), fusA1 (R680C) | |
| ICA01-004 | Sputum | 698 | No | 0.094 | 4 | 1.5 | oprD (D43N), mexE (V156A), ampD (E162Q), armZ (V266M), mexY (D428N) | |
| FQSE11-0603 | CF sputum | 701 | No | 0.125 | 0.38 | 3 | mexB (nt775Δ1), mexY (N709H, A586T), mexX (A38P), oprN (R363H), ampDh2 (P116S) | |
| FQSE11-1010 | CF sputum | 701 | No | 0.5 | 0.75 | ≥256 | aadB, aacA4 | gyrB (R138L), mexY (N709H, A586T), mexX (A38P), oprN (R363H), gyrA (Y267N), nfxB (E75K), ampDh2 (P116S), mpl (Q248X), mexA (nt45Δ1) |
| ARA03-004 | Sputum | 845 | No | 1 | 8 | 4 | gyrA (D87N), ampD (Q131X), mexT (G246S), mexB (nt54Δ1), mexZ (nt302Δ9), dacB (nt781Δ1) | |
| FQSE24-0304 | CF sputum | 1089 | No | 3 | 0.38 | 4 | gyrB (S466F), mexA (L338P), oprM (E456G), oprD (Q67*), mexY (Y355H), mexZ (A194P), galU (P123L), fusA1 (K430E), pmrB (R287Q), mexF (nt1074ins9) | |
| CLE03-006 | Blood | 1337 | No | 3 | 4 | 4 | parR (aa214Δ1), gyrA (D87N), ampD (D59E) | |
| BAL02-001 | Sputum | 1619 | No | 0.047 | 6 | 2 | ||
| NAV01-005 | Sputum | 1637 | No | 0.5 | 0.38 | 12 | gyrB (P749S), mexB (P190L), oprD (nt742Δ1), mexZ (R104W), galU (F248S), fusA1 (Y552C), mexD (L1027V), mexC (V367A), pmrB (A173T), armZ (A262S) | |
| MAD05-008 | Sputum | 1717 | No | 0.5 | 8 | 4 | parS (V152A), mexY (T238I) | |
| BAL04-002 | Blood | 1816 | No | 0.125 | 4 | 1 | mexA (K86E), ampR (G295R), ampC (A278G), parE (E215Q) | |
| BAL02-009 | Sputum | 2124 | No | 1 | 2 | 0.5 | ampDh3 (P55S), mexY (R251H), mexX (A38T), mexZ (G68D), mexS (V104A), ampR (A16V), ampD (T47I), nfxB (R163Q) | |
| GAL02-002 | Sputum | 3342 | No | 0.38 | 8 | 8 | mexZ (Y204X), galU (C242X), mexS (G76S), oprJ (D303V), pmrB (A467V), mexD (nt3068ins9) | |
a
CF, cystic fibrosis; CIP, ciprofloxacin; ATM, aztreonam; TOB, tobramycin; ins, insertion; IS, insertion sequence; X, stop codon. Δ, deletion.