Motility and flagellar examination of P. kilonensis F113 and its fliC mutants. (A) Swimming, swarming, and twitching motilities on KB swim plates (0.3%, 0.5%, and 1.0% agar, respectively). Photographs were taken 24 h (swimming), 48 h (swarming), or 96 h (twitching) after incubation. (B) The quantitative analysis of swimming, swarming, and twitching abilities of P. kilonensis F113 and the fliC mutants. Different letters indicate statistically significant differences between different treatments (one-way ANOVA, Tukey’s test; P < 0.05). (C) Transmission electron microscopy images of the flagella of P. kilonensis F113 and its derivatives. All of the experiments were repeated three times with similar results.