. 2023 Jan 11;11(1):e03621-22. doi: 10.1128/spectrum.03621-22

TABLE 2.

Binding affinity between Flg22 with a single mutation and FLS2 or BAK1

Peptide	Mutated residue	Phase 1 (FLS2-Flg22)		Phase 2 (FLS2/Flg22-BAK1)
Peptide	Mutated residue	ΔΔG_bind (kcal mol⁻¹)	Deleterious^a	ΔΔG_bind (kcal mol⁻¹)	Deleterious^a
Flg22-2	Q1E	1.38	No	−0.44	No
	T5S	0.72	No	−0.65	No
	S7L	−0.13	No	−1.17	No
	R8K	1.37	No	−0.36	No
	K13S	2.22	Yes	–0.3	No
	L19Q	1.37	No	1.52	Yes
	Q20A	2.09	Yes	−0.06	No

Deleterious (yes/no): the MutaBind2 server uses ΔΔG ≥ 1.5 or ≤−1.5 kcal mol⁻¹ to define whether or not a mutation is deleterious. ΔΔG ≥ 1.5 kcal mol⁻¹ is deleterious.