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. 2022 Dec 8;11(1):e03387-22. doi: 10.1128/spectrum.03387-22

FIG 2.

FIG 2

Alpha diversity of different combinations of rumen fluid and solid phases fermented for 24 h in vitro. (A and B) Effects of different bacteria of the rumen digesta on alpha diversity (observed species and Shannon index). (C) The beta diversity of a principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity matrices. Each point represents a unique sample. (D) Venn diagram for the composition of microbiota from fermenters among the eight groups (OTU-level analysis). FFFS = fresh fluid (FF) + fresh solid (FS); FFAS = fresh fluid (FF) + autoclaved solid (AS); FFFW = fresh fluid (FF) + fresh solid washing solution (FW); FFAW = fresh fluid (FF) + autoclaved solid washing solution (AW); AFFS = autoclaved fluid (AF) + fresh solid (FS); AFAS = autoclaved fluid (AF) + autoclaved solid (AS); AFFW = autoclaved fluid (AF) + fresh solid washing solution (FW); and AFAW = autoclaved fluid (AF) + autoclaved solid washing solution (AW).