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. 2022 Dec 8;11(1):e03387-22. doi: 10.1128/spectrum.03387-22

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Copyright © 2022 Zhao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — Functional predictions for microbiota from different bacteria in the rumen digesta of the in vitro fermentation of the diet. (A) Heatmap of KOs, z score. Relative abundance of function pathways: level 1 (B), level 2 (C, top 10), and level 3 (D, top 10 of Carbohydrate Metabolism, Metabolism of Cofactors and Vitamins, and Amino Acid). (E) Featured pathways of different groups by LEfSe. FFFS = fresh fluid (FF) + fresh solid (FS); FFAS = fresh fluid (FF) + autoclaved solid (AS); FFFW = fresh fluid (FF) + fresh solid washing solution (FW); FFAW = fresh fluid (FF) + autoclaved solid washing solution (AW); AFFS = autoclaved fluid (AF) + fresh solid (FS); AFAS = autoclaved fluid (AF) + autoclaved solid (AS); AFFW = autoclaved fluid (AF) + fresh solid washing solution (FW); and AFAW = autoclaved fluid (AF) + autoclaved solid washing solution (AW).