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. 2023 Jan 9;11(1):e03232-22. doi: 10.1128/spectrum.03232-22

TABLE 1.

Kinetic parameters of StlN-ter binding to trimeric Duts determined by BLI

Dut KD (nM) Kon (M−1 s−1) Koff (s−1) R 2 a
mDut 39.25 2.56 × 105 10.00 × 10−3 0.990
mDut H145F 12.46 3.88 × 105 4.83 × 10−3 0.992
hDut 34.44 5.83 × 105 20.20 × 10−3 0.974
ϕ11Dut 7.94 2.84 × 105 2.25 × 10−3 0.990
a

R2 value indicates accuracy between fit and experimental data. Values above 0.95 are considered a good fit.