FIG 1.

Schematic of expression cassettes and immunblots. (A) The pPink HC expression vector with the dual alcohol oxidase (AOX) promoter expression constructs which drive the expression of the P1 capsid protein and the viral protease, 3CD or the expression of protease-free constructs with VP0 (green), VP3 (pink), and VP1 (blue). (B) Immunoblot for PV VP1 and 3D. Samples were collected 48 h postinduction and lysed using 0.1 M NaOH. All samples were boiled in 2x Laemlli buffer and separated by SDS-PAGE prior to analysis by immunoblot using mouse monoclonal α-VP1 and rabbit monoclonal α-3D antibody. The figure is a representative example of three separate experiments for each construct.