FIG 2.

Gradient purification of protease-dependent and protease-free PV-1 SC6b VLPs. All samples were purified by ultracentrifugation and fractioned from top to bottom in 1 mL aliquots. A 12 μL sample from each fraction was then taken and boiled in 5x Laemmli buffer and separated by SDS-PAGE prior to analysis by immunoblot using mouse monoclonal α-VP1. The figure is a representative example of three separate experiments for each construct.