FIG 3.

Antigenicity of PV-1 VLPs. (A) Reactivity of gradient fractions with Mab 234 (D-antigen) and Mab 1588 (C-antigen) in ELISA. The pink dashed line represents the positive control, BRP, for the D-antigen ELISA. OD at λ = 492 nm is represented in arbitrary units. The figure is a representative example of three separate experiments for each construct. (B) Quantification of D-antigen production per 100 mL of expression culture as determined by ELISA with Mab 234 (n = 3). Construct nomenclature is as follows: 31-0 = VP3 P2A VP1 VP0, 30-1 = VP3 P2A VP0 VP1, 03-1 = VP0 P2A VP3 VP1, 01-3 = VP0 P2A VP1 VP3, 10-3 = VP1 P2A VP0 VP3, and 13-0 = VP1 P2A VP3 VP0.