FIG 5.

The effect of RF on C. albicans coenzyme metabolism. (A) Clusters of orthologous groups analysis of downregulated differentially expressed genes was performed, and the main enrichment pathways are indicated by red arrows. (B and C) Schematic model of the endogenous thiamine metabolism pathway (B) and the endogenous riboflavin metabolism pathway (C). Genes encoding proteins in red were upregulated, while genes encoding proteins in green were downregulated. (D) The expression of genes involved in the thiamine and RF metabolic pathways was analyzed. (E) C. albicans cells with or without RF treatment were spotted onto YPD agar plates containing exogenous thiamine or RF. TPP, thiamine pyrophosphate. Data were analyzed by ANOVA (ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).