TABLE 1.
Nonsynonymous mutations in D39 stocks from different laboratoriesa
| Position | Mutation annotation | Gene | Description | Presence of mutation in stock |
|||||
|---|---|---|---|---|---|---|---|---|---|
| D39N | H3 | H65 | H27 | H1 | H44 | ||||
| 657974 | S181L | spxB | Pyruvate oxidase | ||||||
| 157206 | F115L | DQM66_RS00850 | M20/M25/M40 family metallo-hydrolase | ||||||
| 306651 | Y32D | DQM66_RS01685 | ATP-dependent Clp protease ATP-binding subunit | ||||||
| 362142 | N133D | DQM66_RS01950 | PTS mannitol-specific transporter subunit IIBC | ||||||
| 457827 | Y444H | glnA | Type I glutamate-ammonia ligase | ||||||
| 500654 | V323L | DQM66_RS02660 | Valine-tRNA ligase | ||||||
| 500656 | V323L | DQM66_RS02660 | Valine-tRNA ligase | ||||||
| 837501 | A260V | DQM66_RS04395 | LysR family transcriptional regulator | ||||||
| 1019890 | Y91C | DQM66_RS11435 | Hypothetical protein | ||||||
| 1090952 | P274T | DQM66_RS05705 | Polyprenyl synthetase family protein | ||||||
| 1166597 | G237V | DQM66_RS06040 | Carbamoyl phosphate synthase small subunit | ||||||
| 1181971 | A102S | crcB | Fluoride efflux transporter CrcB | ||||||
| 1234414 | A398G | aroA | 3-Phosphoshikimate 1-carboxyvinyltransferase | ||||||
| 1353455 | L274S | atpA | FoF1 ATP synthase subunit alpha | ||||||
| 1366359 | N256S | DQM66_RS07185 | DEAD/DEAH box helicase | ||||||
| 1387760 | D98G | DQM66_RS07305 | NAD(P)H-dependent oxidoreductase | ||||||
| 1511664 | P296A | DQM66_RS07940 | ABC transporter substrate-binding protein | ||||||
| 1565733 | V114F | pknB | Stk1 family PASTA domain-containing Ser/Thr kinase | ||||||
| 1607893 | G53D | DQM66_RS08475 | Asp23/Gls24 family envelope stress response protein | ||||||
| 1735190 | V119A | DQM66_RS09265 | Competence/damage-inducible protein A | ||||||
| 1744924 | S115G | DQM66_RS09325 | M50 family metallopeptidase | ||||||
| 1961957 | A858V | DQM66_RS10510 | Discoidin domain-containing protein | ||||||
| 1978062 | L293P | dltD | d-Alanyl-lipoteichoic acid biosynthesis protein DltD | ||||||
| 2008202 | H39Y | cbpD | Choline binding-anchored murein hydrolase CbpD | ||||||
| 243327 | Y216H | DQM66_RS01355 | LLM class flavin-dependent oxidoreductase | ||||||
| 247387 | D72G | spuA | LPXTG-anchored pullulanase SpuA | ||||||
| 248184 | K56T | rpsL | 30S ribosomal protein S12 | ||||||
| 250241 | I299T | fusA | Elongation factor G | ||||||
| 250522 | A393S | fusA | Elongation factor G | ||||||
| 319145 | A454V | DQM66_RS01735 | Sugar transferase | ||||||
| 342683 | A109V | pbp1a | Penicillin-binding protein PBP1A | ||||||
| 638380 | I218V | DQM66_RS03315 | Amino acid ABC transporter ATP-binding protein | ||||||
| 735779 | V86I | DQM66_RS03875 | Amino acid ABC transporter ATP-binding protein | ||||||
| 753387 | G437D | DQM66_RS03985 | ABC transporter ATP-binding protein | ||||||
| 962360 | R85C | rpmA | 50S ribosomal protein L27 | ||||||
| 1632521 | T232A | pfbA | Multiligand-binding adhesin PfbA | ||||||
| 1721928 | V468E | ply | Cholesterol-dependent cytolysin pneumolysin | ||||||
| 1721997 | L445S | ply | Cholesterol-dependent cytolysin pneumolysin | ||||||
| 1724756 | S23I | DQM66_RS09195 | DUF4231 domain-containing protein | ||||||
| 44870 | S268T | comB | Competence pheromone export protein ComB | ||||||
| 54032 | Q20K | purH | Bifunctional purine biosynthesis protein PurH | ||||||
| 202272 | K65T | rplE | 50S ribosomal protein L5 | ||||||
| 290722 | A303V | DQM66_RS01595 | Sugar kinase | ||||||
| 437741 | F144L | DQM66_RS02330 | ECF-type riboflavin transport substrate binding | ||||||
| 805481 | Q501K | DQM66_RS04210 | DNA polymerase III subunit alpha | ||||||
| 1194993 | Q632* | DQM66_RS06230 | ABC transporter ATP-binding protein | ||||||
| 1676827 | A408D | treP | PTS trehalose-specific EIIBC component | ||||||
| 1681870 | Q206* | DQM66_RS08890 | ABC transporter ATP-binding protein | ||||||
| 1781250 | W334C | DQM66_RS09500 | Bacteriocin-associated integral membrane protein | ||||||
| 215632 | E271D | DQM66_RS01235 | ABC transporter ATP-binding protein | ||||||
| 318872 | P363L | DQM66_RS01735 | Sugar transferase | ||||||
| 643097 | N49D | lctO | l-Lactate oxidase | ||||||
| 1677743 | Q103* | treP | PTS trehalose-specific EIIBC component | ||||||
| 385012 | M129I | DQM66_RS02050 | MarR family transcriptional regulator | ||||||
| 1030176 | Q286R | DQM66_RS05385 | Recombinase family protein | ||||||
| 1466223 | P110S | DQM66_RS07705 | Metallophosphoesterase family protein | ||||||
a
Variant calls were conducted with Breseq using NCTC7466 as the reference. The mutation position is relative to the origin of replication. The mutation annotation column indicates the original amino acid, the position within the protein sequence, and the new amino acid. Shading indicates the presence of the given mutation. PTS, phosphotransferase system. * denotes nonsense mutations resulting in introduction of premature stop codons. LLM is luciferase-like monooxygenase. ECF is energy-coupling factor.