TABLE 2.
Summary of the studies included in the cross-cohort analysisa
| Reference | IBS/HC (no.), age group | DNA/RNA extraction kit or method | Region of 16S rRNA genes/sequence platform |
|---|---|---|---|
| Altomare et al. (10) | 77/86, adults | QIAamp DNA stool minikit (Qiagen, Germany) | V1 to V3/454 Junior GS |
| Labus et al. (27) | 29/23, adults | MO BIO PowerSoil DNA isolation kit (MO BIO Laboratory, CA, USA) | V3 to V5/454 GS |
| Lee et al. (7) | 7/12, adults | QIAamp DNA stool minikits (Qiagen, Venlo, Netherlands) | V3 to V4/Illumina MiSeq |
| Mizuno et al. (28) | 10/10, adults | Enzymatic lysis method using lysozyme (Sigma-Aldrich Co. LCC, Tokyo, Japan) and achromopeptidase (Wako) | V1 and V2/Illumina MiSeq |
| Pozuelo et al. (9) | 202/88, adults | Qiaex II gel extraction kit (Qiagen, Hilden, Germany) | V4/Illumina MiSeq |
| Zhu et al. (6) | 15/14, adults | MO BIO PowerSoil DNA isolation kit (MO BIO Laboratory, CA, USA) | V4/Illumina HiSeq 2500 |
| Zhuang et al. (12) | 20/10, adults | PowerFecal DNA isolation kit (MoBio, Carlsbad, CA, USA) | V3 and V4/Illumina MiSeq |
| Belkova et al. (29) | 10/43, children | Zirconia beads (BeadBug, 0.5-mm zirconium beads, Sigma, USA) and QIAamp DNA stool kit (Qiagen, Germany) | V3 and V4/Illumina HiSeq |
| Saulnier et al. (8) | 178/177, children | MO BIO PowerSoil DNA isolation kit (MO BIO Laboratory, CA, USA) with modifications | V3 to V5/454 GS FLX |
| Our study | 19/24, children | MO BIO PowerSoil DNA isolation kit (MO BIO Laboratory, CA, USA) | V3 and V4/Illumina MiSeq |
a
IBS, irritable bowel syndrome; HC, healthy control.