TABLE 2.
Primers used in PCR amplification
| Primer | Sequence (5′–3′) |
|---|---|
| pCMV-Nat9 | F: CAAGCTTGCGGCCGCGAATTCATGCTGCAGGCTGCTACCAT |
| R: TGCCACCCGGGATCCTCTAGATCAGTGGGGCTCTGAGGACC | |
| pEGFP-Nat9 | F: TCGAGCTCAAGCTTCGAATTCTATGCTGCAGGCTGCTACCAT |
| R: GGATCCCGGGCCCGCGGTACCTCAGTGGGGCTCTGAGGACC | |
| pMal-c5x-Nat9 | F: CGCGATATCGTCGACGGATCCATGCTGCAGGCTGCTACCAT |
| R: ACCTGCAGGGAATTCGGATCCTCAGTGGGGCTCTGAGGACC | |
| pCMV-Nat9 (1-167) | F: CTGCACTTTGAGTGATCTAGAGGATCCC |
| R: CTCAAAGTGCAGCCTGCGGA | |
| pCMV-Nat9 (33-212) | F: GCCGCGAATTCACATGAGTGGATGAAA |
| R: TGAATTCGCGGCCGCAAGA | |
| GP5-Flag | F: CCACACTGGACTAGTGGATCCGCCACCATGTTGGGGAAGTGCTTGAC |
| R: CTTGGTACCGAGCTCGGATCCGAGACGACCCCATTGTTCCG | |
| GP5-Flag (1-88) | F: CAGCCATTTCCTTAAGCTTGGGCCCGACTAC |
| R: AAGGAAATGGCTGGTGGTGA | |
| GP5-Flag (126-200) | F: ATCCGCCACCATGAGGCTTGCGAAGAACTGC |
| R: CATGGTGGCGGATCCACTAG | |
| Nat9-1469/181-Luc | F: ATCTGCGATCTAAGTAAGCTTTCTTACGCCATCCAAGACTAT |
| R: CAGTACCGGAATGCCAAGCTTGAGACAGGCCCAAGGCCTC | |
| Nat9-1049/181-Luc | F: CTAAGTAAGCTTCTCCGGTCTCGGTGA |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-629/181-Luc | F: CTAAGTAAGCTTCACTACAAGGGTCCCCT |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-359/181-Luc | F: CTAAGTAAGCTTTCCCCCCTCCTAGAGAA |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-224/181-Luc | F: CTAAGTAAGCTTCAGCTGTCCCGCAC |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-89/181-Luc | F: CTAAGTAAGCTTGGGACCGGGATTGG |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-14/181-Luc | F: CTAAGTAAGCTTGAGGAAAGGCCGTC |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-0/88-Luc | F: CTAAGTAAGCTTCCCGGGGCTTCAGT |
| R: AAGCTTACTTAGATCGCAGATCTCGA | |
| Nat9-ETV5-Mutant -Luc | F: GTGGCAATAGAGGGCGGGGTGCG |
| R: CCCTCTATTGCCACGCAGACGGCA | |
| Nat9-SP1-Mutant -Luc | F: GAAGAGATCGGGGTGCGGTGCTACG |
| R: CCCCGTACTCTTCCGCCACGCAGAC | |
| Nat9-ASCL1-Mutant-Luc | F: CCGGTGGCAACCGTCTGCGTGGCGG |
| R: CGGTTGCCACCGGGGCCCAATCCCG |