Table 1.
List of new RNA viruses discovered in this study. Read abundances are indicated as the number of reads per million. Likely hosts correspond to eukaryotic lineages detected using BLASTn/BLASTp analyses and phylogenies.
| Virus name | MMETSP sample (phylum/class) | Genome status | Reads/million | BLASTp best hits (GenBank acc./organism) | %ID | e-value | Likely host(s) (BLAST) | Likely host(s) (phylogenies) | Proposed host |
|---|---|---|---|---|---|---|---|---|---|
| Amphitrite narna-like virus | MMETSP1061 P. pungens (Bacillariophyta) |
Full-length | 48 |
QIR30281.1 RdRp [Plasmopara viticola associated narnavirus 2] |
41 | 5e-144 | Bacillariophyta | Fungi/protist | Bacillariophyta |
| Poseidon narna-like virus | MMETSP0418 A. radiata (Bacillariophyta) |
Partial | 8 | QDH89392.1 RdRp, partial [Mitovirus sp.] | 34 | 4e-17 | Bacillariophyta | Marine arthropod | Bacillariophyta |
| Halia narna-like virus | MMETSP0418 A. radiata (Bacillariophyta) |
Full-length | 108 | QBC65281.1 RdRp, partial [Rhizopus microsporus 23S narnavirus] | 32 | 4e-17 | Bacillariophyta | Protist | Bacillariophyta |
| Triton levi-like virus | MMETSP1471 P. provasolii (Chlorophyta) |
Partial | 64 | APG76993.1 hypothetical protein [Beihai levi-like virus 20] | 46 | 3e-65 | Chlorophyta; Bacteria | Bacteria | Bacteria |
| Aiolos mito-like virus | MMETSP0286 P. polylepis (Haptophyta) |
Full-length | 54 |
YP_009272901.1 RdRp [Fusarium poae mitovirus 4] |
35 | 3e-38 | Haptophyta | Sea sponge | Haptophyta |
| Asopus mito-like virus | MMETSP0164 C. braarudii (Haptophyta) |
Partial | 12 |
QDM55307.1 RdRp [Geopora sumneriana mitovirus 1] |
34 | 2e-35 | Haptophyta | Sea sponge | Haptophyta |
| Athena mito-like virus | MMETSP0719 C. curvisetus (Bacillariophyta) |
Partial | 54 | ASM94070.1 putative RdRp, partial [Barns Ness breadcrumb sponge narna-like virus 5] | 65 | 6e-72 | Bacillariophyta; Bacteria | Sea sponge | Bacillariophyta |
| Daimones mito-like virus | MMETSP0286 P. polylepis (Haptophyta) |
Full-length | 104 |
YP_009552787.1 RNA-directed RNA polymerase [Rhizophagus sp. RF1 mitovirus] |
26 | 4e-16 | Haptophyta | Freshwater arthropods | Haptophyta |
| Despoena mito-like virus | MMETSP0167 R. maculata (Rhodophyta) |
Full-length | 115 |
ALM62241.1 RdRp [Soybean leaf-associated mitovirus 1] |
34 | 6e-32 | Rhodophyta; Bacteria | Freshwater arthropods | Rhodophyta |
| Proteus mito-like virus | MMETSP1081 P. amylifera (Chlorophyta) |
Full-length | 388 |
ALM62242.1 RdRp [Soybean leaf-associated mitovirus 2] |
32 | 7e-46 | Chlorophyta | Fungi/protist | Chlorophyta |
| Telchines mito-like virus | MMETSP0725 Amphiprora (Bacillariophyta) |
Partial | 15 |
QDA33961.1 RdRp [Mitovirus 1 BEG47] |
25 | 5e-21 | Bacillariophyta | Algae | Bacillariophyta |
| MMETSP0724 Amphiprora (Bacillariophyta) |
Partial | 26 | |||||||
| Susy yue-like virus | MMETSP1423 P. heimii (Bacillariophyta) |
Partial | 5 | QDH86724.1 RdRp, partial [Qinviridae sp.] | 42 | 1e-21 | Bacillariophyta | Soil samples/marine arthropod | Bacillariophyta |
| Aethusa amalga-like virus | MMETSP0011 R. marinus (Rhodophyta) |
Partial | 83 |
ANN12897.1 putative CP/RdRp [Zygosaccharomyces bailii virus Z] |
43 | 2e-12 | Rhodophyta; Bacteria | Marine arthropod | Rhodophyta |
| Benthesicyme durna-like virus | MMETSP1319 T. pacifica (Bolidophyceae) |
Partial | 404 | QDH90748.1 RdRp, partial [Partitiviridae sp.] | 29 | 1e-17 | Bolidophyceae | Protist | Bolidophyceae |
| Herophile durna-like virus | MMETSP0140 P. australis (Bacillariophyta) |
Partial | 10 |
QOW97238.1 RdRp [Amalga-like lacheneauvirus] |
27 | 2e-19 | Bacillariophyta | Chlorophyta | Bacillariophyta |
| Cymopoleia durna-like virus | MMETSP1081 P. amylifera (Chlorophyta) |
Partial | 10 |
YP_009551448.1 RdRp [Diatom colony-associated dsRNA virus 2] |
31 | 2e-34 | Chlorophyta | Fungi | Chlorophyta |
| Ourea durna-like virus | MMETSP0797 D. acuminata (Dinophyceae) |
Partial | 4 |
ARO72610.1 RdRp [Spinach deltapartitivirus 1] |
27 | 4e-11 | Dinophyceae; Bacteria | Land plant | Dinophyceae |
| Aegean partiti-like virus | MMETSP0491 T. chuii (Chlorophyta) |
Full-length | 3,296 |
QOW97235.1 RdRp [Partiti-like lacotivirus] |
29 | 6e-62 | Chlorophyta | Chlorophyta | Chlorophyta |
| Pelias marna-like virus | MMETSP1377 Symbiodinium sp. (Dinophyceae) |
Full-length | 60,553 |
YP_009337401.1 hypothetical protein 2 [Wenzhou picorna-like virus 4] |
26 | 8e-98 | Dinophyceae | Algae | Xanthophyceae |
| Neleus marna-like virus, 1 | MMETSP0946 V. litorea (Xanthophyceae) |
Full-length | 806,763 |
YP_009336927.1 hypothetical protein 1 [Shahe picorna-like virus 3] |
33 | 3e-180 | Vaucheriaceae | Algae | Xanthophyceae |
| Neleus marna-like virus, 2 | MMETSP0945 V. litorea (Xanthophyceae) |
Full-length | 711,119 |
YP_009336927.1 hypothetical protein 1 [Shahe picorna-like virus 3] |
33 | 4e-180 | Vaucheriaceae | Algae | Xanthophyceae |
| Tyro marna-like virus | MMETSP0905 T. antarctica (Bacillariophyta) |
Partial | 126 |
YP_001429582.1 hypothetical protein JP-A_gp2 [Marine RNA virus JP-A] |
75 | 3e-272 | Bacillariophyta; Bacteria | Algae | Bacillariophyta |
| MMETSP0903 T. antarctica (Bacillariophyta) |
Partial | 2,034 | |||||||
| MMETSP0902 T. antarctica (Bacillariophyta) |
Partial | 237 | |||||||
| Aloadae toti-like virus, 1 | MMETSP1388 Isochrysis (Haptophyta) |
Partial | 39 |
QIJ70132.1 RdRp [Keenan toti-like virus] |
33 | 2e-109 | Haptophyta | Fungi /invertebrates |
Haptophyta |
| Aloadae toti-like virus, 2 | MMETSP1090 Isochrysis (Haptophyta) |
Partial | 11 |
QIJ70132.1 RdRp [Keenan toti-like virus] |
33 | 2e-109 | Haptophyta | Fungi /invertebrates |
Haptophyta |
| Antaeus toti-like virus, 1 | MMETSP0154 T. antarctica (Bacillariophyta) |
Full-length | 27 | QGY72637.1 putative CP [Plasmopara viticola associated totivirus-like 2] | 22 | 1e-10 | Bacillariophyta | Protist | Bacillariophyta |
| Antaeus toti-like virus, 2 | MMETSP0152 T. antarctica (Bacillariophyta) |
Full-length | 7 |
BBJ21451.1 CP-RdRp fusion protein [Pythium splendens RNA virus 1] |
40 | 5e-53 | Bacillariophyta | Protist | Bacillariophyta |
| Charybdis toti-like virus | MMETSP0853 P. fraudulenta (Bacillariophyta) |
Partial | 38 |
YP_003288763.1 RdRp [Rosellinia necatrix megabirnavirus 1/W779] |
30 | 4e-24 | Bacillariophyta; Bacteria | Fungi | Bacillariophyta |
| MMETSP0851 P. fraudulenta (Bacillariophyta) |
Partial | 44 | |||||||
| MMETSP0850 P. fraudulenta (Bacillariophyta) |
Partial | 41 | |||||||
| MMETSP0852 P. fraudulenta (Bacillariophyta) |
Partial | 14 | |||||||
| Chrysaor toti-like virus | MMETSP0418 A. radiata (Bacillariophyta) |
Partial | 40 |
YP_009551502.1 RdRp [Diatom colony-associated dsRNA virus 17 genome type B] |
27 | 9e-95 | Bacillariophyta; Bacteria | Soil | Bacillariophyta |
| Laestrygon toti-like virus | MMETSP1451 V. brassicaformis (Chromeraceae) |
Partial | 29 |
YP_009551504.1 RdRp [Diatom colony-associated dsRNA virus 17 genome type A] |
34 | 4e-112 | Chromeraceae | Soil | Chromeraceae |
| Arion toti-like virus | MMETSP0796 P. bahamense (Dinophyceae) |
Partial | 31 |
QGA70930.1 RdRp [Ahus virus] |
25 | 3e-18 | Dinophyceae; Bacteria | Protist/ marine host | Dinophyceae |
| Otus toti-like virus | MMETSP0011 R. marinus (Rhodophyta) |
Full-length | 31 |
AMB17469.1 RdRp, partial [Delisea pulchra totivirus IndA] |
51 | 3e-120 | Rhodophyta; Bacteria | Fungi | Rhodophyta |
| Polyphemus toti-like virus | MMETSP0418 A. radiata (Bacillariophyta) |
Partial | 10 |
YP_009552789.1 RdRp [Diatom colony-associated dsRNA virus 5] |
59 | 3e-79 | Bacillariophyta; Bacteria | Algae/ protist | Bacillariophyta |
| Ephialtes toti-like virus | MMETSP0418 A. radiata (Bacillariophyta) |
Partial | 13 |
YP_009552789.1 RdRp [diatom colony-associated dsRNA virus 5] |
63 | 1e-200 | Bacillariophyta; Bacteria | Algae/ protist | Bacillariophyta |