FIGURE 3.

Validation of ubiquitous and specific markers of MSC‐derived apoVs. (a, b) Heatmaps showing the differentially expressed proteins (DEPs) of the four categories (a) and apoptotic markers (b) in hBMSC‐derived apoVs compared to exosomes. Category 1: Transmembrane or GPI‐anchored proteins associated to plasma membrane and/or endosomes; Category 2: Cytosolic proteins recovered in EVs; Category 3: Ribosomal proteins; Category 4: Transmembrane, lipid‐bound and soluble proteins associated to other intracellular compartments than PM/endosomes. Rows represent proteins and columns represent individual replicates. (c) Multiple reaction monitoring (MRM) analysis of candidate proteins for hBMSC‐derived apoV biomarkers. (d) Western blotting analysis showing the inclusive (13 proteins) and exclusive (Syntenin‐1) biomarkers of apoVs from hBMSCs and hASCs. (e) Protein‐protein interaction (PPI) network analysis of the ubiquitous and specific apoV proteins. (f) Representation of the putative apoV markers identified in MSC‐derived apoVs. Exos, exosomes. hBMSCs, human bone marrow MSCs; hASCs, human adipose MSCs