Figure 4. Slug deficiency in LepRb cells induces energy imbalance.

Mice were fed a chow diet. (A) Food intake at 9 weeks of age. Male Slug^fl/fl, n = 5; male Slug^ΔLepRb, n = 5; female Slug^fl/fl, n = 4; female Slug^ΔLepRb, n = 4. (B) Body core temperatures at 9–10 weeks of age. Male Slug^fl/fl, n = 5; male Slug^ΔLepRb, n = 5; female Slug^fl/fl, n = 4; female Slug^ΔLepRb, n = 4. (C–E) O₂ consumption, CO₂ production (normalized to lean mass), and respiratory exchange ratio (RER) at 9 weeks of age (n = 4 mice per group). Data are presented as mean ± SEM. *P < 0.05, 2-tailed unpaired Student’s t test.