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. 2023 Feb 15;133(4):e162434. doi: 10.1172/JCI162434

Figure 1. Manipulation of UBE2C, but not UBE2S, affects the proliferation and survival of lung cancer cells.

Figure 1

(A and B) A427 (A) and H1792 (B) cells were transfected with siRNA targeting UBE2C or control (siCont) for 24 hours. Cells were then seeded in 96-well plates in triplicate and analyzed with a CCK-8 cell proliferation assay. (C and D) A427 (C) and H1792 (D) cells were transfected with siRNA targeting UBE2C, UBE2S, or siCont for 24 hours, followed by clonogenic survival assay. Representative pictures were taken (top) and colonies were counted and are shown as mean ± SEM (n = 3) (bottom). (E) MEFs were infected with Ad-GFP or Ad-Cre for 72 hours and analyzed by CCK-8 cell proliferation assay or IB (inset). (F and G) H1792 (F) and H358 (G) cells were transfected with plasmid expressing FLAG-tagged UBE2C or vector control for 48 hours and analyzed by CCK-8 cell proliferation assay or IB (inset). (H–J) A427 (H), H23 (I), and H1792 (J) cells were transfected with siRNA targeting UBE2C or siCont for 48 hours, followed by FACS analysis. (K) H1792 and A427 cells were transfected with siRNA targeting UBE2C or siCont for 48 hours, followed by IB with indicated antibodies. *P < 0.05; **P < 0.01 by 2-tailed Student’s t test (A, B, and E–J) or 1-way ANOVA test (C and D).