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. 2023 Feb 15;133(4):e162434. doi: 10.1172/JCI162434

Figure 2. Deletion of Ube2c, but not Ube2s, inhibits lung tumorigenesis induced by KrasG12D, and extends the lifespan of mice.

Figure 2

(A) H1650 cells were transfected with plasmid pLVX-KrasG12D-V5, followed by IB with indicated antibodies (top), or by RT-qPCR analysis (bottom). (B) Ube2c deletion remarkably reduced lung tumor burden in the KrasG12D mouse model. The lung tissues were isolated from mice with indicated genotypes, fixed, sectioned, and stained with H&E. (C) Quantification of mouse lung tumors in mice with indicated genotypes after Ad-Cre administration. The lesions (hyperplasia and lung tumors) in all 5 lobes of lung tissues were counted (n = 10 per group). (D) Kaplan-Meier survival curves of mice with indicated genotypes and number for up to 30 weeks after administration of Ad-Cre. (E) The lung tissues from mice with indicated genotypes were fixed, sectioned, and stained with H&E. (F) Kaplan-Meier survival curves of mice with indicated genotypes and number for up to 36 weeks after administration of Ad-Cre. *P < 0.05; **P < 0.01 by 1-way ANOVA test (A), 2-tailed Student’s t test (C and E), or the log-rank test (D and F). Scale bars: 20 μm.