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. 2023 Feb 15;133(4):e162434. doi: 10.1172/JCI162434

Figure 3. UBE2C differentially regulates mTORC signaling and DEPTOR levels.

Figure 3

(A) H1792 and A427 cells were transfected with siRNA targeting UBE2C or siCont for 48 hours, followed by IB with indicated antibodies. (B) H1792 and H358 cells were transfected with plasmids of FLAG-UBE2C or vector for 48 hours, followed by IB with indicated antibodies. (C) Ube2c deletion inactivates mTORC1/2 signals. The lung tissues were isolated from mice with indicated genotypes, fixed and sectioned, and stained by IHC with the indicated antibodies. The staining quantification was analyzed by a semiquantitative immunoreactivity scoring system, as described in the Methods section. *P < 0.05, **P < 0.01 by 2-tailed Student’s t test (C). Scale bar: 50 μm.