Skip to main content
. 2023 Feb 15;133(4):e162434. doi: 10.1172/JCI162434

Figure 6. DEPTOR knockdown rescues the phenotypes induced by UBE2C knockdown in lung cancer cells.

Figure 6

(A and B) A427 (A) and H1792 (B) cells were transfected with indicated siRNAs for 24 hours; one portion of cells was analyzed by IB (inset), and the other portion was seeded in 96-well plates with medium containing 2% FBS in triplicate and analyzed by CCK-8 cell proliferation assay on the indicated days. (C) A427 and H1792 cells were transfected with the indicated siRNAs for 24 hours, followed by clonogenic survival assay. A representative dish from each indicated group was photographed (left) and colonies were counted and are shown as mean ± SEM (n = 3) (right). (D) H1792 and A427 cells were transfected with indicated siRNAs for 48 hours, followed by IB with indicated antibodies. (E) Knockdown of UBE2C or CDH1 induced autophagy, which was rescued by DEPTOR knockdown. H358 cell were transfected with indicated siRNAs for 48 hours and stained with the indicated Abs, followed by photography under a fluorescence microscope (left). Data are the mean ± SEM of 4 independent experiments (right). *P < 0.05; **P < 0.01 by 1-way ANOVA test (A–C and E). Scale bar: 20 μm.