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. 2023 Feb 15;133(4):e160807. doi: 10.1172/JCI160807

Figure 1. CTCL is associated with tumor growth, immune cell accumulation, and chronic itch.

Figure 1

(A) Time course of tumor growth in CTCL mice. n = 8–18. One-way ANOVA, F(8, 115) = 22.36, P < 0.0001. (B) Time course of scratching bouts in CTCL mice. n = 7. One-way ANOVA, F(8, 54) = 22.61, P < 0.0001. (C) Time course of alloknesis in CTCL mice. n = 8. One-way ANOVA, F(7, 56) = 68, P < 0.0001. Alloknesis was induced by an innocuous von Frey filament (bending force = 0.07 g). (D) Representative image of lymphoma on day 20. Scale bar: 0.5 cm. (E) Representative images of DAPI immunostaining of the back skin of naive and CTCL mice at day 20. Dotted lines show the epidermis. Scale bar: 40 μm. (F) Time course of the thickness of the epidermis of CTCL mice. n = 3. One-way ANOVA, F(7, 16) = 50.07, P < 0.0001. (G) Representative images of toluidine blue staining for mast cells in the back skin of naive and CTCL mice at day 20. Dotted lines show the epidermis. Scale bar: 50 μm. (H) Time course showing the number of mast cells in lymphoma tumors. n = 3. One-way ANOVA, F(7, 16) = 14.07, P < 0.0001. (I–K) Time course showing the number of CD68+ macrophages (H), Gr-1+ neutrophils (I), and CD11C+ DCs (J). n = 3. One-way ANOVA, (H) F(7, 16) = 8.56, P = 0.0002; (I) F(7, 16) = 29.54, P < 0.0001; and (J) F(7, 16) = 60.65, P < 0.0001. Data are expressed as the mean ± SEM. One-way ANOVA with Bonferroni’s post hoc test, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus the naive group.