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. 2023 Feb 15;133(4):e160807. doi: 10.1172/JCI160807

Figure 2. CTCL results in nerve injury in the late phases.

Figure 2

(A) Double immunostaining for ATF3 and IB4 in cervical DRGs of naive and CTCL mice at day 60. Scale bar: 25 μm. (B) Double immunostaining of ATF3 and the neuronal markers NF200, P2X3, and CGRP in cervical DRGs from CTCL mice at day 60. Scale bar: 25 μm. (C) Percentage of ATF3+, IB4+, NF200+, P2X3+, and CGRP+ neurons in the cervical DRG of CTCL mice at different time points. n = 3. Two-way ANOVA, F(12, 30) = 9.15, P < 0.0001. (D) Percentage of ATF3 colocalization with P2X3+ neurons in the cervical DRG of CTCL mice at different time points. n = 3. One-way ANOVA, F(3, 8) = 31.89, P < 0.0001. (E) Pie chart showing ATF3 colocalization with NF200+, P2X3+, and CGRP+ neurons in the cervical DRG from a CTCL mouse on day 60. Data were collected from 3 animals. (F) Double immunostaining for CGRP and IB4 in the cervical spinal cord dorsal horn from CTCL mice at day 60. Arrowheads indicate the loss of IB4+ primary afferents in the IIi. Scale bar: 100 μm. Data are expressed as the mean ± SEM. One- or 2-way ANOVA with Bonferroni’s post hoc test, **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus the naive group.