Figure 10. TF cytoplasmic domain phosphorylation–dependent increased TGF-β1 activation in clinical setting of MI.

(A) Representative confocal images of phosphorylation status of TF in infarcted myocardium obtained from WT or TF^ΔCT mice after 7 days. Representative images and quantification of biological replicates. Kruskal-Wallis test and Dunn’s multiple-comparison test; n = 3–4 animals per group. Scale bars: 50 μm. (B) Representative immunofluorescence confocal microscopy images of CD45⁺ and CD45/p-TF double-positive cells in human myocardium specimens obtained from n = 5 nonischemic (NI) donor hearts and n = 7 IHF patients. Quantification of biological replicates. Mann-Whitney test. Scale bars: 50 μm. (C and D) Western blot analysis and quantification of human LV tissue obtained from n = 5 nonischemic donor hearts and n = 9 IHF patients for p-TF (normalized to total TF) and TF (C) or TGF-β1 (normalized to GAPDH) and p-SMAD2 (normalized to total SMAD2) (D). Mann-Whitney test. Data are shown as mean ± SEM. *P < 0.05.