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. 2023 Feb 15;133(4):e162870. doi: 10.1172/JCI162870

Figure 10. SHP2 activation in CD47/SIRPα axis of TIMs is related to the prognosis of CRC patients.

Figure 10

(A–D) Macrophages of human tissue were collected (n = 16). mRNA levels are shown (A). SHP2 was IP with normalized protein concentration, and phosphatase activity was quantified (B). Correlations between SENP8 mRNA and SHP2 phosphatase activity in TIMs (C). Correlations between deneddylation level and SHP2 phosphatase activity in TIMs (D). (E) Expression of SIRPα in tumor-infiltrating myeloid cells between CRC samples. n = 12 (NAC-treatment naive samples); n = 8 (NAC-treated PR samples); and n = 5 (NAC-treated PD/SD samples). (F) Rate of SIRPα+ and SHP2+ cells among TIMs. (G) Correlation of macrophage infiltration score and TNM stage of COAD cohort from the TCGA database. n = 234 (I and II); n = 183 (III and IV). (H) Proposed model of SHP2 deneddylation to ensure CD47/SIPRα signal. Data are represented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Two-tailed, unpaired Student’s t test (A and B); Pearson’s correlation (C and D); Wilcoxon’s test (E and G).