Figure 1. Epithelial HDAC3 expression limits commensal-specific CD4⁺ T cells in the intestine.

(A and B) Number of cBir1⁺ tetramer-specific CD4⁺ T cells isolated from large intestine of neonatal GF and CNV pups. (C) mRNA expression of HDAC3 in IECs isolated from large intestine of GF and CNV pups. (D and E) Frequency of total intestinal CD3⁺. (F and G) Frequency of total intestinal CD8a⁺ (F) and CD4⁺ (G) T cells. (H–K) Number of cBir1⁺ tetramer-specific CD4⁺ T cells (H and I) and frequency of RORγt⁺ (J) and FoxP3⁺ (K) cBir1⁺CD4⁺ T cells in large intestine of HDAC3^FF and HDAC3^ΔIEC mice. cBir1⁺ tetramer cells are gated on live, CD45⁺, lineage (CD11b^–B220^–Ly6G^–, CD11c^–CD8a^–, CD4⁺. Data are representative of at least 2 experiments, 3–4 mice per group. *P < 0.05, **P < 0.01, ****P < 0.0001, by 1-way ANOVA with Tukey’s multiple-comparison test (B) or unpaired 2-tailed Student’s t test (C–K).