Figure 3. HDAC3 regulates expression of MHCII on IECs.

(A) Frequency of total MHCII⁺ cells in colon lamina propria. (B and C) Frequency of dendritic cells and macrophages in large intestinal lamina propria of HDAC3^FF and HDAC3^ΔIEC mice. Gated on live, CD45⁺, MHCII⁺. (D) MHCII⁺ cells at colonic luminal surface. (E) Frequency of total MHCII⁺ cells in D. (F and G) Frequency of MHCII⁺ EpCAM⁺ cells in large intestine of GF and CNV mice. (H) mRNA expression of H2-Ab1 in IECs isolated from large intestine of GF and CNV pups. (I and J) Frequency of MHCII⁺ EpCAM⁺ cells in large intestine of HDAC3^FF and HDAC3^ΔIEC mice. (K and L) H2-Ab1 (K) and CIITA (L) mRNA in IECs isolated from the large intestine of HDAC3^FF and HDAC3^ΔIEC mice. (M) HDAC3 mRNA in HDAC3^FF and HDAC3^ΔIEC-IND organoids treated with tamoxifen (4-OHT). (N) H2-Ab1 mRNA expression in HDAC3^FF and HDAC3^ΔIEC-IND organoids treated with IKK-16. Data are representative of at least 3 independent experiments, 3–5 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by unpaired 2-tailed Student’s t test (E–M) or 1-way ANOVA with Tukey’s multiple-comparison test (N).