Figure 5. HDAC3 enables microbiota to regulate epithelium-dependent, commensal-specific immunity.

(A) Frequency of rectal prolapse in control (CNV) and antibiotic-treated (ABX) MHCII^FF and MHCII^ΔIEC mice. (B and C) Frequency of RORγt⁺ cBir1⁺-specific CD4⁺ T cells (B) and IL-17 mRNA expression (C) in large intestine of control and ABX-treated MHCII^FF and MHCII^ΔIEC mice. (D and E) Frequency of MHCII⁺ EpCAM⁺ cells in large intestine of GF- and CNV- HDAC3^FF and HDAC3^ΔIEC mice. (F–H) Number of cBir1⁺ tetramer-specific CD4⁺ T cells (F) and frequency of FoxP3⁺ (G) and RORγt⁺ (H) cBir1⁺ T cells isolated from GF- and CNV-HDAC3^FF and HDAC3^ΔIEC mice. cBir1⁺ tetramer cells are gated on live, CD45⁺, lineage (CD11b^–B220^–Ly6G^–, CD11c^–CD8a^–), CD4⁺. Data are representative of at least 2 independent experiments, 3–5 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by Mantel-Cox (A) or 1-way ANOVA with Tukey’s multiple-comparison test (B–H).