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[Preprint]. 2023 Jun 14:2023.01.26.525752. Originally published 2023 Jan 27. [Version 2] doi: 10.1101/2023.01.26.525752

Figure 2. MR antagonists produce different MR quaternary configurations.

Figure 2.

Inset shows representative images of single cell nuclei expressing MR-GFP and treated with 1 μM spironolactone (Spiro), 1 μM eplerenone (Eple) or 1 μM finerenone. White arrows point to the MMTV array. Scale bars: 5 μm. Plot shows MR molecular brightness (ε) assessed using the N&B technique. To facilitate comparison, data from Fig.1 showing ε for GR-N525 and MR treated with 10 nM aldosterone are included. Data points correspond to ε obtained from a single nucleus (n = 490, 307, 82, 47, 55, 24, 37, 24, 19, 11, 41 and 33 cells in each condition, from left to right). Horizontal bars represent mean ± 95% CI. Each ε value was compared to its reference value (MR/10 nM Aldo in the nucleoplasm or at the MMTV array) using the Kruskal-Wallis test followed by Dunńs multiple comparisons correction. Symbols refer to statistical differences with MR/10 nM Aldo in the same compartment (nucleoplasm or MMTV array; **, p<0.01).