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[Preprint]. 2023 Jun 14:2023.01.26.525752. Originally published 2023 Jan 27. [Version 2] doi: 10.1101/2023.01.26.525752

Figure 4. The NTD is essential for MR tetramerization in the nucleoplasm.

Figure 4.

(A) Schematic representation of MR deletion and domain swapping constructs. NTD, N-terminal domain; DBD, DNA binding domain; LBD, ligand binding domain. (B) Representative images of single cell nuclei expressing the indicated constructs. White arrows point to the MMTV array. Scale bars: 5 μm. (C) N&B results obtained with the indicated constructs. To facilitate comparison, data from Fig.1 showing ε for GR-N525 and MR treated with 10 nM aldosterone are included. Data points correspond to ε obtained from a single nucleus (n = 490, 307, 82, 47, 42, 22, 43, 22, 35 and 23 cells in each condition, from left to right). Horizontal bars represent mean ± 95% CI. Each ε value was compared to its reference value (wild type MR in the nucleoplasm or at the MMTV array) using the Kruskal-Wallis test followed by Dunńs multiple comparisons correction. Symbols refer to statistical differences with MR wild type in the same compartment (nucleoplasm or MMTV array; n.s., non-significant; **, p<0.01).