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. 2023 Feb 14;21(2):e3002000. doi: 10.1371/journal.pbio.3002000

Fig 1. Separately housed Cd4creAhrfl/fl recover from EAE with increased myelin staining at chronic phase.

Fig 1

(A) Clinical score of Cd4creAhrfl/fl and Ahrfl/fl mice cohoused (females; representative plot includes n = 9 mice/group; total replicates of N = 2 experiments). Spinal cords sections of Cd4creAhrfl/fl and Ahrfl/fl were stained with Luxol fast blue and hematoxylin/eosin stain at day 31 post EAE induction (B) Representative images and (C) quantification of myelin stain. Images from 4 equally spaced spinal cord levels were averaged for each mouse. (n = 5 mice/group; unpaired t test p = 0.0706) (D) Clinical score of Cd4creAhrfl/fl and Ahrfl/fl littermate controls separated at weaning (3 weeks of age). (Females; representative plot includes n = 8–9 mice/group; total replicates of N = 2 experiments; Mann–Whitney U test on total scores reported in legend [p = 0.0096] and on single days reported on plot) (E) Luxol fast blue with hematoxylin/eosin stain at the peak stage of EAE (day 16) and at chronic phase (day 31). (F) Quantification of myelin stain by Luxol fast blue alone in Ahrfl/fl mice and (G) Cd4creAhrfl/fl mice. (n = 7–8 mice/group; N = 2 experiments; unpaired t tests [p = 0.8343, 0.0322]) Scale bars represent 400 μm. Error bars represent standard error from the mean. Raw data can be found in Supporting information (S1 Data). EAE, experimental autoimmune encephalomyelitis.