Fig 2. RsmQ interacts with its preferred binding sequence (GGA/AnGGA) and this interaction is mediated by the VHRE/D binding site.
(a) Percentage Rmax values for RsmQ WT (green) H43A (blue) and R44A (red) binding to ssDNA containing the binding sites shown above. Two-way ANOVA showed an effect of both binding site (p < 0.0001) and mutation (p < 0.0001) on Rmax. (b) Percentage Rmax values for WT RsmQ binding to ssDNAs containing the above binding site. Binding sites that are predicted to bind RsmQ are shown in purple. All oligos are designed as hairpins and results shown are for RsmQ at a concentration of 100 nM. Error bars represent the standard deviation of 2 replicates. Data are available in S1 Data. ssDNA, single-stranded DNA; WT, wild-type.