Fig 7. NOD1 signaling in C. trachomatis infected reporter cells is increased under YkfC inhibitor treatment.

NOD1 reporter cells were infected with C. trachomatis D and used to analyze abundance of NOD1 activating PGN-derived material shed by the intracellular pathogen in the presence of YkfC_Ctr inhibitor chloroacetone. Infected NOD1 reporter cells were treated 6 hpi with chloroacetone and analyzed 18 hpi (a) and 24 hpi (b) resulting in treatment durations of 12 h and 18 h, respectively. An increase of NOD1 activation was observed at concentrations (0.016 and 0.031 μg/mL) below both cytotoxicity towards the reporter cell line (IC₅₀ 15 μg/mL after 24 h treatment, JA Fig in S1 Text) and the MIC of chloroacetone towards C. trachomatis (4 μg/mL, observed after treatment durations of 6, 20 and 24 hpi, Fig 5). Addition of TriDAP 6 hpi was used as a positive control for NOD1 activation. Error bars indicate ± s.d. (n = 3). Ns: not significant. ***P-value ≤0.0001.