Appendix 1—table 1. List of polymerase chain reaction primer sequences used in mRNA expression analysis.
| Gene | Forward primer (5’–3’) | Reverse primer (5’–3’) |
|---|---|---|
| Abcg5 | GAGCTGCAGAGGATGATTGCT | AGCCACCCTGGTCTTGGA |
| Acta2 | CCTGACGGGCAGGTGATC | ATGAAAGATGGCTGGAAGAGAGTCT |
| Actb | AACCGTGAAAAGATGACCCAGAT | CACAGCCTGGATGGCTACGTA |
| Adgre1 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Adipoq | CTCCACCCAAGGGAACTTGT | TAGGACCAAGAAGACCTGCATC |
| Apob | GCCCATTGTGGACAAGTTGATC | CCAGGACTTGGAGGTCTTGGA |
| Ccl2 | GCATCTGCCCTAAGGTCTTCA | TTCACTGTCACACTGGTCACTCCTA |
| Cd36 | GCAAAGAACAGCAGCAAAATC | CAGTGAAGGCTCAAAGATGG |
| Col1a1 | GAGAGAGCATGACCGATGGATT | TGTAGGCTACGCTGTTCTTGCA |
| Cpt1a | GAGACTTCCAACGCATGACA | ATGGGTTGGGGTGATGTAGA |
| Ctgf | GGCCTCTTCTGCGATTTCG | CCATCTTTGGCAGTGCACACT |
| Cyp7a1 | CAGGGAGATGCTCTGTGTTCA | AGGCATACATCCCTTCCGTGA |
| Cyp8b1 | GGACAGCCTATCCTTGGTGA | CGGAACTTCCTGAACAGCTC |
| Cyp27a1 | TCTGGCTACCTGCACTTCCT | CTGGATCTCTGGGCTCTTTG |
| Codon-optimized Fgf21 | GCCCACCTGGAGATCAGGGAGGA | GGCAGGAAGCGCACAGGTCCCCAG |
| Fgf21 | GGGGTCATTCAAATCCTGGGTGTCA | ACACATTGTAACCGTCCTCCAGCAG |
| Fgfr1 | AGAGTCCAAGAGTAAAAGCAGC | CTTCCGAGGTTCAGCTCTCC |
| Fgfr2 | GCTATAAGGTACGAAACCAGCAC | GGTTGATGGACCCGTATTCATTC |
| Fgfr4 | TCCATGACCGTCGTACACAAT | ATTTGACAGTATTCCCGGCAG |
| Il1b | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| Klb | TGTTCTGCTGCGAGCTGTTAC | TACCGGACTCACGTACTGTTT |
| Mttp | CTCTTGGCAGTGCTTTTTCTCT | GAGCTTGTATAGCCGCTCATT |
| Pgc1a | TGCTAGCGGTTCTCACAGAG | AGTGCTAAGACCGCTGCATT |
| Ppara | ATGCCAGTACTGCCGTTTTC | GGCCTTGACCTTGTTCATGT |
| Rplp0 | GGACCCGAGAAGACCTCCTT | GCACATCACTCAGAATTTCAATGG |
| Tgfb1 | TTGCCCTCTACAACCAACACAA | GGCTTGCGACCCACGTAGTA |
| Tnfa | AGCCCACGTCGTAGCAAACCAC | TCGGGGCAGCCTTGTCCCTT |
Abcg5, ATP-binding cassette transporter G member 5; Acta2, actin α2; Actb, β-actin Adgre1, adhesion G protein-coupled receptor E1; Adipoq, adiponectin; Apob, apolipoprotein B; Ccl2, C–C motif chemokine ligand 2; Cd36, cluster of differentiation 36; Col1a1, collagen type 1α1; Cpt1a, carnitine palmitoyl transferase 1α; Ctgf, connective tissue growth factor; Cyp7a1, cholesterol 7α-hydroxylase; Cyp8b1, sterol 12α-hydroxylase; Cyp27a1, sterol 27-hydroxylase; Fgf21, exogenous fibroblast growth factor; Fgfr, fibroblast growth factor receptor; Il1b, interleukin-1β; Klb, β-Klotho; Pgc1a, peroxisome proliferator-activated receptor gamma coactivator 1α; Ppara, peroxisome proliferator-activated receptor α; Rplp0, ribosomal protein lateral stalk subunit p0; Tgfb1, transforming growth factor-β; Tnfa, tumor necrosis factor α.